Sodium citrate blood collection tubes are intended to yield inside ± 10% of the expressed draw volume on the name of the added substance tube all through the whole timeframe of realistic usability.
CLSI suggests that sodium citrate tubes be spun at 1500 RCF for 15 minutes to attain platelet poor plasma (platelet check < 10, 000) and precise coagulation test outcomes. The scratched fill marker on the plastic citrate tubes shows the base worthy blood volume in the tube. Robotized instruments oblige a normal of 100 µl for every test for PT and APTT, 50 µl for Fibrinogen, and 20 µl for Factor Assays.
There have been late research articles demonstrating that drawing a toss tube is not important before drawing the sodium citrate tube. In the CLSI coagulation record (H21-A5) it is still proposed that the citrate tube is the second or third tube drawn. Then again, in the CLSI rule for Venipuncture Collection (H3-A6) it states that for routine PT and APTT, the first tube drawn may be utilized for testing.
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